Cell-free microRNAs in kidney graft preservation fluid as novel biomarker for delayed graft function

H.P. Roest, L.S.S. Ooms, A.J.M. Gillis, J.N.M. IJzermans, L.H.J. Looijenga, F.J.M.F. Dor, L.J.W. van der Laan

Friday 16 march 2018

11:30 - 11:40h at Van Weelde Zaal

Categories: Basic / translational research, Session (parallel)

Parallel session: Parallel session 12: Basic/translational research

Background

Delayed graft function is a common complication after deceased donor kidney transplantation (KTx), which affects both short and long-term outcome. Currently available biomarkers in graft preservation fluid lack sensitivity in predicting outcome after transplantation. The aim of this study is to identify microRNAs in preservation fluid predictive of delayed graft function (DGF) after transplantation.

Methods

In this study, preservation samples were collected during kidney transplantations from deceased donors. The graft outcome was defined as DGF or immediate graft function (IF). As a discovery cohort 4 IF samples and 4 DGF samples were analysed using Taqman Array MicroRNA cards with 2 grafts from both DCD (donation after cardiac death) and DBD (donation after brain death) in each group. As validation cohort, we analysed 40 IF and DGF samples.

Results

On average, 222 miRNAs (range 192-246) were detected per sample with 223 miRNAs fulfilling the pre-set parameters (Ct

Discussion

MiRNAs in graft preservation fluids are well detectable and can be a promising source for biomarkers of graft quality and for predicting outcome prior to kidney transplantation. In the era of extended criteria donor organs, miR-505 guided graft reconditioning strategies may help to improve transplant outcome.